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htert tigf  (ATCC)


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    Structured Review

    ATCC htert tigf
    S-0636 exhibits a low potential of cytotoxicity in human cell lines. Human liver cells (HepG2, black bars), human embryonic kidney cells (HEK293, grey bars), human gingival keratinocytes (hTERT-TIGK, dark grey bars), and human gingival fibroblasts <t>(hTERT-TIGF,</t> light gray bars) were seeded and cultivated as described above. Once the cells reached ∼80% confluency, the medium was replaced with fresh medium containing various concentrations of S-0636, followed by an additional 24-hour incubation under the same conditions. The WST-8 assay determined the viability of cells. Cells treated with 0.1% Triton X-100 served as a negative control), while cells treated with the S-0636 compound diluent served as a positive control. Cell viability was quantified by measuring the absorbance of WST-8 formazan at 450 nM using a Tecan Sunrise microplate reader. All experiments were performed in at least three independent biological replicates.
    Htert Tigf, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htert+tigf/hTERT+Gingival+Fibroblast/pmc13021670-49-10-19
    Average 94 stars, based on 24 article reviews
    htert tigf - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Novel small molecule targeting PgQC reduces Porphyromonas gingivalis virulence"

    Article Title: Novel small molecule targeting PgQC reduces Porphyromonas gingivalis virulence

    Journal: Frontiers in Oral Health

    doi: 10.3389/froh.2026.1716188

    S-0636 exhibits a low potential of cytotoxicity in human cell lines. Human liver cells (HepG2, black bars), human embryonic kidney cells (HEK293, grey bars), human gingival keratinocytes (hTERT-TIGK, dark grey bars), and human gingival fibroblasts (hTERT-TIGF, light gray bars) were seeded and cultivated as described above. Once the cells reached ∼80% confluency, the medium was replaced with fresh medium containing various concentrations of S-0636, followed by an additional 24-hour incubation under the same conditions. The WST-8 assay determined the viability of cells. Cells treated with 0.1% Triton X-100 served as a negative control), while cells treated with the S-0636 compound diluent served as a positive control. Cell viability was quantified by measuring the absorbance of WST-8 formazan at 450 nM using a Tecan Sunrise microplate reader. All experiments were performed in at least three independent biological replicates.
    Figure Legend Snippet: S-0636 exhibits a low potential of cytotoxicity in human cell lines. Human liver cells (HepG2, black bars), human embryonic kidney cells (HEK293, grey bars), human gingival keratinocytes (hTERT-TIGK, dark grey bars), and human gingival fibroblasts (hTERT-TIGF, light gray bars) were seeded and cultivated as described above. Once the cells reached ∼80% confluency, the medium was replaced with fresh medium containing various concentrations of S-0636, followed by an additional 24-hour incubation under the same conditions. The WST-8 assay determined the viability of cells. Cells treated with 0.1% Triton X-100 served as a negative control), while cells treated with the S-0636 compound diluent served as a positive control. Cell viability was quantified by measuring the absorbance of WST-8 formazan at 450 nM using a Tecan Sunrise microplate reader. All experiments were performed in at least three independent biological replicates.

    Techniques Used: Incubation, Negative Control, Positive Control

    Related Articles

    Cell Culture:

    Article Title: Novel small molecule targeting PgQC reduces Porphyromonas gingivalis virulence
    Article Snippet: .. The cell lines hTERT-TIGK (human telomerase immortalized gingival keratinocytes) and hTERT-TIGF (human telomerase immortalized gingival fibroblasts) were obtained from American Type Culture Collection (ATCC,CRL-3397). hTERT-TIGK cells were cultured in GibcoTM Medium 154 supplemented with the Human Keratinocyte Growth Kit (Gibco). hTERT-TIGF cells were maintained in Dulbecco's Modified Eagle Medium (DMEM/F12) supplemented with 10% fetal bovine serum (FBS) and GlutaMAXTM (Gibco). ..

    Article Title: Novel small molecule targeting PgQC reduces Porphyromonas gingivalis virulence
    Article Snippet: .. The cell lines hTERT-TIGK (human telomerase immortalized gingival keratinocytes) and hTERT-TIGF (human telomerase immortalized gingival fibroblasts) were obtained from American Type Culture Collection (ATCC,CRL-3397). hTERT-TIGK cells were cultured in GibcoTM Medium 154 supplemented with the Human Keratinocyte Growth Kit (Gibco). hTERT-TIGF cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM/F12) supplemented with 10% fetal bovine serum (FBS) and GlutaMAXTM (Gibco). ..

    Modification:

    Article Title: Novel small molecule targeting PgQC reduces Porphyromonas gingivalis virulence
    Article Snippet: .. The cell lines hTERT-TIGK (human telomerase immortalized gingival keratinocytes) and hTERT-TIGF (human telomerase immortalized gingival fibroblasts) were obtained from American Type Culture Collection (ATCC,CRL-3397). hTERT-TIGK cells were cultured in GibcoTM Medium 154 supplemented with the Human Keratinocyte Growth Kit (Gibco). hTERT-TIGF cells were maintained in Dulbecco's Modified Eagle Medium (DMEM/F12) supplemented with 10% fetal bovine serum (FBS) and GlutaMAXTM (Gibco). ..

    Article Title: Novel small molecule targeting PgQC reduces Porphyromonas gingivalis virulence
    Article Snippet: .. The cell lines hTERT-TIGK (human telomerase immortalized gingival keratinocytes) and hTERT-TIGF (human telomerase immortalized gingival fibroblasts) were obtained from American Type Culture Collection (ATCC,CRL-3397). hTERT-TIGK cells were cultured in GibcoTM Medium 154 supplemented with the Human Keratinocyte Growth Kit (Gibco). hTERT-TIGF cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM/F12) supplemented with 10% fetal bovine serum (FBS) and GlutaMAXTM (Gibco). ..



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    htert tigf  (ATCC)
    94
    ATCC htert tigf
    S-0636 exhibits a low potential of cytotoxicity in human cell lines. Human liver cells (HepG2, black bars), human embryonic kidney cells (HEK293, grey bars), human gingival keratinocytes (hTERT-TIGK, dark grey bars), and human gingival fibroblasts <t>(hTERT-TIGF,</t> light gray bars) were seeded and cultivated as described above. Once the cells reached ∼80% confluency, the medium was replaced with fresh medium containing various concentrations of S-0636, followed by an additional 24-hour incubation under the same conditions. The WST-8 assay determined the viability of cells. Cells treated with 0.1% Triton X-100 served as a negative control), while cells treated with the S-0636 compound diluent served as a positive control. Cell viability was quantified by measuring the absorbance of WST-8 formazan at 450 nM using a Tecan Sunrise microplate reader. All experiments were performed in at least three independent biological replicates.
    Htert Tigf, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htert+tigf/hTERT+Gingival+Fibroblast/pmc13021670-49-10-19
    Average 94 stars, based on 1 article reviews
    htert tigf - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    S-0636 exhibits a low potential of cytotoxicity in human cell lines. Human liver cells (HepG2, black bars), human embryonic kidney cells (HEK293, grey bars), human gingival keratinocytes (hTERT-TIGK, dark grey bars), and human gingival fibroblasts (hTERT-TIGF, light gray bars) were seeded and cultivated as described above. Once the cells reached ∼80% confluency, the medium was replaced with fresh medium containing various concentrations of S-0636, followed by an additional 24-hour incubation under the same conditions. The WST-8 assay determined the viability of cells. Cells treated with 0.1% Triton X-100 served as a negative control), while cells treated with the S-0636 compound diluent served as a positive control. Cell viability was quantified by measuring the absorbance of WST-8 formazan at 450 nM using a Tecan Sunrise microplate reader. All experiments were performed in at least three independent biological replicates.

    Journal: Frontiers in Oral Health

    Article Title: Novel small molecule targeting PgQC reduces Porphyromonas gingivalis virulence

    doi: 10.3389/froh.2026.1716188

    Figure Lengend Snippet: S-0636 exhibits a low potential of cytotoxicity in human cell lines. Human liver cells (HepG2, black bars), human embryonic kidney cells (HEK293, grey bars), human gingival keratinocytes (hTERT-TIGK, dark grey bars), and human gingival fibroblasts (hTERT-TIGF, light gray bars) were seeded and cultivated as described above. Once the cells reached ∼80% confluency, the medium was replaced with fresh medium containing various concentrations of S-0636, followed by an additional 24-hour incubation under the same conditions. The WST-8 assay determined the viability of cells. Cells treated with 0.1% Triton X-100 served as a negative control), while cells treated with the S-0636 compound diluent served as a positive control. Cell viability was quantified by measuring the absorbance of WST-8 formazan at 450 nM using a Tecan Sunrise microplate reader. All experiments were performed in at least three independent biological replicates.

    Article Snippet: The cell lines hTERT-TIGK (human telomerase immortalized gingival keratinocytes) and hTERT-TIGF (human telomerase immortalized gingival fibroblasts) were obtained from American Type Culture Collection (ATCC,CRL-3397). hTERT-TIGK cells were cultured in GibcoTM Medium 154 supplemented with the Human Keratinocyte Growth Kit (Gibco). hTERT-TIGF cells were maintained in Dulbecco's Modified Eagle Medium (DMEM/F12) supplemented with 10% fetal bovine serum (FBS) and GlutaMAXTM (Gibco).

    Techniques: Incubation, Negative Control, Positive Control